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InviScreen® GMO Detection Kit (35S/NOS/FMV)

Item No.: 6014001201

Highlights:

1. Screening of genetically modified DNA sequences according to ISO 21569
2. Target sequences are present in >85 % of approved GMOs
3. Compatible with open platform qPCR thermocyclers
4. User-friendly PCR reaction setup in just 2 pipetting steps

Product Details

The InviScreen® GMO Detection Kit (35S/NOS/FMV) provides a real-time PCR method for the screening of GMO DNA sequences in food and feed. The kit combines the ISO 21569 real-time PCR screening method, based on the detection of the CaMV 35S promoter and the NOS terminator genes, with the detection of the FMV promoter. The pCaMV 35S and tNOS are often present, simultaneous or individually, in many genetically modified plants, and therefore may be used to monitor the presence of a wide range of first-generation GMOs. Complementary to this, the detection of pFMV provides further information regarding genetically modified plants.

The kit includes a plant-specific primer set to control the entire analytical process from nucleic acid extraction to DNA amplification. The real-time PCR method can be performed in commonly available PCR instruments with FAM and VIC/HEX fluorescence channels. Under optimal conditions, the kit provides highly sensitive detection of at least 0.1% relative GMO content.

Sample preparation	For DNA extraction the use of InviSorb® Spin Food Kit or InviSorb® Spin Plant Mini Kit are recommended.
LOD (Detection Limit)	0.1%
Specificity	Cauliflower mosaic virus (CaMV) 35S promoter Agrobacterium tumefaciens nopaline synthase (NOS) terminator Figwort mosaic virus 34S (FMV) promoter Plant DNA
No. of tests	50
Technology	qPCR / Real-Time PCR
Instrument compatibility	Applied Biosystems™ 7500, Agilent AriaMx™, Bio Rad CFX96 Touch™ and CFX96 Touch™ Deep Well
Dyes	Channels FAM and HEX
Certification	RUO